RT Journal Article
SR Electronic
T1 Haplotypes of (−794(CATT)<sub>5–8</sub>/−173G&gt;C) <em>MIF</em> gene polymorphisms and its soluble levels in basal cell carcinoma in western Mexican population
JF Journal of Investigative Medicine
JO J Investig Med
FD BMJ Publishing Group Ltd
SP 41
OP 46
DO 10.1136/jim-2020-001414
VO 69
IS 1
A1 Guevara-Gutiérrez, Elizabeth
A1 Castro-Jonguitud, María José
A1 De la Torre-Flores, Susana Elizabeth
A1 Muñoz-Valle, José Francisco
A1 Tlacuilo-Parra, Alberto
A1 Salazar-Torres, Francisco Javier
A1 Valle, Yeminia
A1 Padilla-Gutiérrez, Jorge Ramón
A1 Martínez-Fernández, Diana Emilia
A1 Valdés-Alvarado, Emmanuel
YR 2021
UL http://jim.bmj.com/content/69/1/41.abstract
AB Basal cell carcinoma (BCC) is the most common dermatological neoplasms in Caucasian populations. In Mexico, a prevalence of 3.9 per 1000 habitants is estimated. Recently, the macrophage migration inhibitory factor (MIF) has been related to different types of cancer. Therefore, this study aimed to investigate the genetic association of haplotypes of [-794(CATT)5-8/-173G&gt;C]MIF gene polymorphisms and its soluble levels in BCC. A total of 360 individuals were recruited for the study, that is, 180 of the total amounts were patients with BCC histologically confirmed and the remaining 180 individuals were identified as control subjects (CS). Both polymorphisms were genotyped by PCR and PCR-RFLP (restriction fragment length polymorphism), and MIF serum levels were measured by ELISA kit. A borderline difference was found between the 55 genotype and the susceptibility to BCC (5.6% vs 1.7% in BCC and CS, respectively, OR=3.7 and p=0.04). Furthermore, the haplotype 7G showed a significant association with BCC (p=0.02, OR=1.99). Concerning MIF soluble levels, patients with BCC showed a media of 2.1 ng/mL and CS showed 4.4 ng/mL, the comparison between groups was significant (p&lt;0.01). Our findings suggest that the 55 genotype and the haplotype 7G are associated with the susceptibility to BCC; furthermore, a significant difference was found between MIF soluble levels in both study groups.