PT  - JOURNAL ARTICLE
AU  - Guevara-Gutiérrez, Elizabeth
AU  - Castro-Jonguitud, María José
AU  - De la Torre-Flores, Susana Elizabeth
AU  - Muñoz-Valle, José Francisco
AU  - Tlacuilo-Parra, Alberto
AU  - Salazar-Torres, Francisco Javier
AU  - Valle, Yeminia
AU  - Padilla-Gutiérrez, Jorge Ramón
AU  - Martínez-Fernández, Diana Emilia
AU  - Valdés-Alvarado, Emmanuel
TI  - Haplotypes of (−794(CATT)<sub>5–8</sub>/−173G>C) <em>MIF</em> gene polymorphisms and its soluble levels in basal cell carcinoma in western Mexican population
AID  - 10.1136/jim-2020-001414
DP  - 2021 Jan 01
TA  - Journal of Investigative Medicine
PG  - 41--46
VI  - 69
IP  - 1
4099  - http://jim.bmj.com/content/69/1/41.short
4100  - http://jim.bmj.com/content/69/1/41.full
SO  - J Investig Med2021 Jan 01; 69
AB  - Basal cell carcinoma (BCC) is the most common dermatological neoplasms in Caucasian populations. In Mexico, a prevalence of 3.9 per 1000 habitants is estimated. Recently, the macrophage migration inhibitory factor (MIF) has been related to different types of cancer. Therefore, this study aimed to investigate the genetic association of haplotypes of [-794(CATT)5-8/-173G>C]MIF gene polymorphisms and its soluble levels in BCC. A total of 360 individuals were recruited for the study, that is, 180 of the total amounts were patients with BCC histologically confirmed and the remaining 180 individuals were identified as control subjects (CS). Both polymorphisms were genotyped by PCR and PCR-RFLP (restriction fragment length polymorphism), and MIF serum levels were measured by ELISA kit. A borderline difference was found between the 55 genotype and the susceptibility to BCC (5.6% vs 1.7% in BCC and CS, respectively, OR=3.7 and p=0.04). Furthermore, the haplotype 7G showed a significant association with BCC (p=0.02, OR=1.99). Concerning MIF soluble levels, patients with BCC showed a media of 2.1 ng/mL and CS showed 4.4 ng/mL, the comparison between groups was significant (p<0.01). Our findings suggest that the 55 genotype and the haplotype 7G are associated with the susceptibility to BCC; furthermore, a significant difference was found between MIF soluble levels in both study groups.