The objective of this study was to determine the relationship between renal injury and inflammatory response induced by high-fat diet in rabbits and the interventional effect of allisartan. Fifteen 6-week-old healthy male rabbits were randomly divided into three groups: normal control (NC) group, high-lipid diet (HLD) group, high-lipid diet and allisartan (HLD+ALST) group. After allisartan treatment for 12 weeks, changes in total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), serum creatinine (Scr) and blood urea nitrogen (BUN) were measured enzymatically in the three groups. The left side of the kidney tissue was kept for paraffin section, and HE staining, periodic acid-Schiff (PAS) staining and Masson staining were used to observe the renal pathologic changes. TC, TG, LDL-C, Scr and BUN levels were all higher and HDL-C levels were lower in the HLD group compared with the NC group. Compared with the HLD group, Scr and BUN levels were significantly decreased in the HLD+ALST group. The results of HE staining showed that allisartan improved the changes of renal tissue morphology in rabbits on high-fat diet, reduced glomerular mesangial cell proliferation and improved glomerulosclerosis; PAS staining showed that glomerular glycogen deposition was reduced and glomerular red staining was significantly lighter; Masson staining showed that renal tubular blue-stained collagen fibers were reduced. In conclusion, hyperlipidemia can lead to aberrant expression of multiple cellular proteins and kidney tissue morphological damage in rabbits. On the other hand, allisartan attenuated renal injury and the mechanism may be related to the downregulation of the inflammatory response.
Data availability statement
Data are available upon reasonable request.
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Correction notice This article has been corrected since it was published Online First. The funding statement has been updated.
Contributors LRB and LL have made substantial contributions to the conception and design of the study. ZJD and WD were involved in acquisition of data, data entry and data cleaning. CXR and BL were involved in analysis and interpretation of data. LRB and ZL have been involved in drafting the manuscript. YXH was involved in revising manuscript critically for important intellectual content. All authors contributed substantially to its revision. All authors read and approved the final manuscript. YXH is the guarantor of this study.
Funding This study was supported by the S&T Program of Hebei Province, China (No. 22377749D).
Competing interests None declared.
Provenance and peer review Not commissioned; externally peer reviewed.