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Depletion of death-associated protein-3 induces chemoresistance in gastric cancer cells through the β-catenin/LGR5/Bcl-2 axis
  1. Yongning Jia1,
  2. Ziyu Li1,
  3. Xiaojing Cheng2,
  4. Xiaojiang Wu1,
  5. Fei Pang1,
  6. Jinyao Shi1,
  7. Shen Li1,
  8. Xiaolong Li1,
  9. Ying Hu3,
  10. Lianhai Zhang1,3,
  11. Jiafu Ji1,3
  1. 1 Department of Gastrointestinal Surgery, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Beijing, China
  2. 2 Department of Gastrointestinal Translational Research, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Beijing, China
  3. 3 Tissue Bank, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Beijing, China
  1. Correspondence to Dr Jiafu Ji, Department of Gastrointestinal Surgery, Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital & Institute, Beijing 100142, China; jijiafu{at}hsc.pku.edu.cn

Abstract

Previously, we demonstrated that death-associated protein-3 (DAP3) loss drives chemoresistance in gastric cancer cells. In the present study, we aimed to determine the underlying molecular mechanism. The effect of DAP3 silencing on β-catenin signaling was assessed. The direct mediator of DAP3 silencing-induced chemoresistance was identified. Depletion of DAP3 stimulates nuclear accumulation of β-catenin and enhances β-catenin-dependent transcriptional activity in gastric cancer cells. However, the protein kinase B , , extracellular regulated protein kinase and signal transducer and activator of transcription 3 signaling pathways remain unaffected by DAP3 loss. We found that the downstream target gene LGR5 (leucine-rich G-protein coupled receptor 5) is upregulated in DAP3-depleted gastric cancer cells. Moreover, knockdown of LGR5 resensitizes DAP3-depleted gastric cancer cells to 5-fluorouracil (5-FU) and oxaliplatin. We also observed that ectopic expression of LGR5 reduces apoptosis in gastric cancer cells on treatment with 5-FU and oxaliplatin, which is accompanied by prevention of caspase-3 cleavage. The antiapoptotic protein Bcl-2 is identified as a key mediator of LGR5-induced apoptosis resistance in gastric cancer cells. The present findings indicate that DAP3 deficiency-induced chemoresistance in gastric cancer is at least partially mediated through the β-catenin/LGR5/Bcl-2 axis. Targeting LGR5 may provide a novel strategy to overcome chemoresistance in DAP3-deficient gastric cancer cells.

  • cell death
  • cancer

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Footnotes

  • YJ and ZL contributed equally.

  • Contributors YJ, LZ, YH, and JJ participated in study design, data collection, and drafting of the manuscript. ZL, XC, XW, FP, JS, SL, and XL conducted the experiments.

  • Funding This work was supported by the National Natural Science Foundation of China (Grant No 81502643), the Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support (ZYLX201701), the National Key Technology Support Program (No 2014BA109B02), the Open Fund of Beijing Key Laboratory of Tumor Invasion and Metastasis (Grant Nos 1150170658 and 2015ZLQX03), Beijing Municipal Administration of Hospitals’Youth Programme (QML20171107), and the Open Fund of Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Peking University Cancer Hospital and Institute of China.

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Patient consent for publication Not required.

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