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NanoSIMS imaging: an approach for visualizing and quantifying lipids in cells and tissues
  1. Cuiwen He1,
  2. Loren G Fong1,
  3. Stephen G Young1,2,
  4. Haibo Jiang3
  1. 1Departments of Medicine, David Geffen School of Medicine, University of California, Los Angeles, California, USA
  2. 2Departments of Human Genetics, David Geffen School of Medicine, University of California, Los Angeles, California, USA
  3. 3Centre for Microscopy, Characterisation, and Analysis, The University of Western Australia, Perth, Western Australia, Australia
  1. Correspondence to Dr Haibo Jiang, Centre for Microscopy, Characterisation, and Analysis, The University of Western Australia, Perth, WA 6009, Australia; haibo.jiang{at}uwa.edu.au

Abstract

Over the past few decades, several approaches have been used to image lipids in cells and tissues, but most have limited spatial resolution and sensitivity. Here, we discuss a relatively new approach, nanoscale secondary ion mass spectrometry imaging, that makes it possible to visualize lipids in cells and tissues in a quantitative fashion and with high spatial resolution and high sensitivity.

  • Lipids

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Footnotes

  • Funding National Heart, Lung, and Blood Institute (F32HL132471-01; HL090553; HL125335).

  • Competing interests None declared.

  • Provenance and peer review Commissioned; externally peer reviewed.

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