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Network analysis of genes associated with esophageal squamous cell carcinoma progression
  1. Xu Li1,
  2. Zhenkun Xia2
  1. 1 The First Department of Thoracic Surgery, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Changsha, China
  2. 2 Department of Thoracic Surgery, Second Xiangya Hospital, Changsha, China
  1. Correspondence to Dr Zhenkun Xia, Second Xiangya Hospital, Changsha, Hunan 410011, China; xiazhenkun0924{at}


This study aims to identify possible genes associated with esophageal squamous cell carcinoma (ESCC) by bioinformatics tool and further explore the function of immunoglobulin heavy chain variable family 4 gene (IGHV4)-28 in the ESCC progression.

The ESCC-related genes in Cancer Genome Atlas (TCGA) database were analyzed by bioinformatics tools, which finally identified IGHV4-28. The expression levels of IGHV4-28 in TE-4 and EC9706 cells were detected by quantitative reverse transcription-PCR (qRT-PCR). Then oe-IGHV4-28 or sh-IGHV4-28 was transfected into TE-4 and EC9706 cells to verify the effect on cell proliferation, migration, invasion, and apoptosis rate. In vivo, a nude mouse model of ESCC was developed, whereby the tumor volume and weight were calculated to evaluate the impact of IGHV4-8 on tumor growth.

Bioinformatics analysis using TCGA database showed that IGHV4-28, IGLV6-57, and KPRP were all associated with ESCC progression. Kaplan-Meier (KM) analysis showed overexpression of IGHV4-28 is substantially associated with the survival rate of patients with ESCC. IGHV4-28 was highly expressed in TE-4 and EC9706 cell lines and overexpression of IGHV4-28 enhanced cell proliferation, invasion, and migration, as well as decreased apoptosis rate. Moreover, nude mice transplanted with IGHV4-28-silencing TE-4 cells showed restrained tumor weight and volume.

In summary, IGHV4-28 was increasingly expressed in ESCC and may serve as a therapeutic target in the treatment of ESCC.

  • 25-hydroxyvitamin D 2

Data availability statement

The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.

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Data availability statement

The datasets used or analyzed during the current study are available from the corresponding author on reasonable request.

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  • Contributors ZX and XL conceived the ideas. ZX and XL designed the experiments. XL performed the experiments. ZX and XL analyzed the data. ZX provided critical materials. XL wrote the manuscript. ZX supervised the study. All the authors have read and approved the final version for publication. ZX is responsible for the overall content as guarantor. ZX accepts full responsibility for the finished work and the conduct of the study, had access to the data, and controlled the decision to publish.

  • Funding The authors have not declared a specific grant for this research from any funding agency in the public, commercial or not-for-profit sectors.

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.