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Antidiabetic effect of Sophora pachycarpa seeds extract in streptozotocin-induced diabetic mice: a statistical evaluation
  1. Behnam Mahdavi1,
  2. Toktam Hajar2,
  3. Alireza Ghodsi3,
  4. Majid Mohammadhosseini4,
  5. Mohammad Mehmandost1,
  6. Elahe Talebi1
  1. 1 Department of Chemistry, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran
  2. 2 Department of Biology, Faculty of Science, Hakim Sabzevari University, Sabzevar, Iran
  3. 3 Department of Statistics, Faculty of Mathematics and Computer Science, Hakim Sabzevari Univrersity, Sabzevar, Iran
  4. 4 Department of Chemistry, College of Basic Sciences, Shahrood Branch, Islamic Azad University, Shahrood, Iran
  1. Correspondence to Dr Behnam Mahdavi, Chemistry, Hakim Sabzevari University, 9617976487 Sabzevar, Iran; b.mahdavi{at}


Undoubtedly, identification of the chemical composition of organic extracts or secondary metabolites of plant materials and evaluation of their potential bioactivity are among the main objectives of natural products-based investigations. In the present study, we report the chemical composition and antidiabetic activity of Sophora pachycarpa (Family Fabaceae) seeds extract (SPE) for the first time. First, the plant seeds were macerated in ethanol. The extract was subjected to analysis on a gas chromatography-mass spectrometry (GC-MS) system to identify the chemical composition. In vivo assay was run to evaluate the antidiabetic activity of the extract. Forty mice were divided into four groups, namely healthy mice, untreated diabetic mice, diabetic mice treated with metformin and diabetic mice treated with SPE. The antidiabetic activity of SPE was analyzed using three statistical methods, namely analysis of variance, K-means, and principal component analysis. According to GC-MS analysis, alkaloids of sophoridine, oleic acid, linoleic acid, and n-hexadecanoic acid were among the most abundant constituent components of SPE. The extract also exhibited a notable antidiabetic activity and remarkably decreased the levels of alkaline phosphatase (ALP), serum glutamic pyruvic transaminase (SGPT), and serum glutamic oxaloacetic transaminase (SGOT) enzymes. The statistical analyses revealed there are no significant differences between the ability of SPE and metformin in the regulation of fasting blood sugar level and liver enzymes (ALP, SGPT, and SGOT). A quinolizidine alkaloid, namely sophoridine, along with fatty acids, viz oleic, linoleic, and n-hexadecanoic acid, were characterized as the major compounds in S. tachycardia seeds extract. The plant extract was also found as a potent agent to reduce blood glucose and liver enzymes.

  • diabetes complications
  • mass spectrometry
  • blood glucose

Data availability statement

Data sharing is not applicable to this article as no new data were created or analyzed in this study.

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Data availability statement

Data sharing is not applicable to this article as no new data were created or analyzed in this study.

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  • Contributors BM: conceptualization, supervision, original draft preparation. TH: methodology, original draft preparation. AG: statistical software, analyses, interpretation of data. MMo: writing-reviewing and editing. MMe and ET ran the laboratory experimentals.

  • Funding The authors have not declared a specific grant for this research from any funding agency in the public, commercial or not-for-profit sectors.

  • Competing interests None declared.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Supplemental material This content has been supplied by the author(s). It has not been vetted by BMJ Publishing Group Limited (BMJ) and may not have been peer-reviewed. Any opinions or recommendations discussed are solely those of the author(s) and are not endorsed by BMJ. BMJ disclaims all liability and responsibility arising from any reliance placed on the content. Where the content includes any translated material, BMJ does not warrant the accuracy and reliability of the translations (including but not limited to local regulations, clinical guidelines, terminology, drug names and drug dosages), and is not responsible for any error and/or omissions arising from translation and adaptation or otherwise.