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MiR-3150b-3p inhibits the progression of colorectal cancer cells via targeting GOLPH3
  1. Weiqing Zhang1,
  2. Xiaoyan Chen2,
  3. Junzhi Jia2
  1. 1 Department of General Surgery, Wuwei People’s Hospital, Wuwei, Gansu, China
  2. 2 Day Care Ward, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot, China
  1. Correspondence to Dr Junzhi Jia, Day Care Ward, The Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010050, China; junzhijia_12{at}126.com

Abstract

The aim of this study was to investigate the function of miR-3150b-3p in malignant behaviors of colorectal cancer (CRC). The tumor-inhibitive effect of miR-3150b-3p was determined by cell viability, invasion, and migration assays. The influence of miR-3150b-3p on the expression of Golgi phosphoprotein 3 (GOLPH3) and Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) pathway was evaluated by luciferase reporter, qRT-PCR and western blot analysis. MiR-3150b-3p was markedly decreased in CRC cell lines compared with colonic mucosal epithelial cell line (FHC). Furthermore, miR-3150b-3p inhibited malignant biological behaviors by targeting GOLPH3, an oncogene in CRC. Additionally, we suggested that miR-3150b-3p ameliorated CRC tumorigenesis in vitro through GOLPH3-mediated JAK2/STAT3 pathway. MiR-3150b-3p might function as a promising tumor suppressor in CRC.

  • colorectal cancer
  • GOLPH3
  • JAK2/STAT3
  • miR-3150b-3p
  • tumor suppressor
https://creativecommons.org/licenses/by/4.0/

This is an open access article distributed in accordance with the Creative Commons Attribution 4.0 Unported (CC BY 4.0) license, which permits others to copy, redistribute, remix, transform and build upon this work for any purpose, provided the original work is properly cited, a link to the licence is given, and indication of whether changes were made. See: https://creativecommons.org/licenses/by/4.0/.

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Footnotes

  • WZ and XC contributed equally.

  • Contributors WZ and XC designed the study, conducted most of the experiments and wrote the manuscript. JJ conducted the experiments and analyzed the data.

  • Funding The authors have not declared a specific grant for this research from any funding agency in the public, commercial or not-for-profit sectors.

  • Competing interests None declared.

  • Patient consent for publication Not required.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement Data are available upon reasonable request.

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