Article Text

Download PDFPDF
Implication of alpha-Klotho as the predictive factor of stress
  1. Kaori Nakanishi,
  2. Makoto Nishida,
  3. Manabu Taneike,
  4. Ryohei Yamamoto,
  5. Hiroyoshi Adachi,
  6. Toshiki Moriyama,
  7. Keiko Yamauchi-Takihara
  1. Health and Counseling Center, Osaka University, Toyonaka, Osaka, Japan
  1. Correspondence to Dr Keiko Yamauchi-Takihara, Health and Counseling Center, Osaka University, Toyonaka, Osaka, Japan; takihara{at}


Stress is known as a risk factor for both mental and physical health problems. While stress is known as one of the major health problems in modern society, a biomarker of stress has not yet been well established. In the present study, we focused on the serum levels of α-Klotho (αKl) as a possible objective biomarker of stress. Subjects included apparently healthy individuals who underwent a health examination in the Osaka University Health and Counseling Center. Physical and biochemical parameters were obtained from all subjects. Information regarding the lifestyle of each individual was obtained via questionnaires. Among male subjects, serum levels of soluble αKl (sαKl) were significantly elevated in subjects who had poor stress management and unsatisfactory sleep, suggesting that stress management and sleeping conditions influenced the serum levels of sαKl. The total Kessler Screening Scale for Psychological Distress (K6) score was significantly increased in subjects who reported experiencing considerable stress, had poor stress management and unsatisfactory sleep. Since serum levels of sαKl showed the same tendency as the K6 score in terms of the relationship between stress management and sleeping conditions in male subjects, increased sαKl levels could be associated with considerable psychological stress in healthy men.

  • psychological stress
  • biomarker
  • α-Klotho
  • interleukin-6
  • kessler screening scale for psychological distress

Statistics from


  • Contributors KN, MN and KY-T designed the study. KN performed the experiments, analyzed the data and wrote the manuscript. MN, HA and KY-T provided appropriate suggestions during the development of this study. Valuable comments on a first draft were received from MT, RY and TM. All authors have given approval to the final version of the manuscript.

  • Funding This work was supported by JSPS KAKENHI (Grant Number JP15K16515, JP15K01712, JP18K17923).

  • Competing interests No, there are no competing interests for any author.

  • Ethics approval All procedures in this study were performed in accordance with the Declaration of Helsinki and the ethics guidelines for clinical research from the Ministry of Health, Labour and Welfare and the Ministry of Education, Culture, Sports, Science and Technology, Japan. All experimental protocols in this study were approved by the Ethics Committee of Health and Counseling Center, Osaka University.

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data sharing statement All data relevant to the study are included in the article.

  • Patient consent for publication Not required.

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.