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Changes in calcium channel proteins according to magnesium sulfate administration in placentas from pregnancies with pre-eclampsia or fetal growth restriction
  1. Hyun-Hwa Cha1,
  2. Jae-Ryoung Hwang2,
  3. Ji Hee Sung3,
  4. Suk-Joo Choi4,
  5. Soo-young Oh4,
  6. Cheong-Rae Roh4
  1. 1 Department of Obstetrics and Gynecology, Kyungpook National University Hospital, School of Medicine, Kyungpook National University, Daegu, Korea
  2. 2 Sungkynkwan University School of Medicine, Samsung Biomedical Research Institute, Samsung Medical Center, Seoul, Korea
  3. 3 Department of Obstetrics and Gynecology, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea
  4. 4 Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea
  1. Correspondence to Dr Cheong-Rae Roh, Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 135-710, Korea; crroh{at}skku.edu

Abstract

We aimed to evaluate the changes in plasma membrane Ca2+-ATPase (PMCA) and sarcoendoplasmic reticulum CA2+-ATPase (SERCA-2) according to the antepartal magnesium sulfate (MgSO4) administration in the placentas from pregnancies with pre-eclampsia (PE) or fetal growth restriction (FGR). Pregnant women were classified as follows: (group 1) pregnancies without PE or FGR (n=16), (group 2) pregnancies with PE or FGR but without MgSO4 administration (n=14), and (group 3) pregnancies with PE or FGR and with MgSO4 administration (n=28). We observed the localization of PMCA and SERCA-2 in placentas and compared its expression among 3 groups. And we observed its expression in BeWo cells following treatment with MgSO4 and CoCl2. PMCA staining was more observed in the basal membrane, whereas SERCA-2 staining was observed predominantly under the microvillous membrane. SERCA-2 expression was significantly increased in group 3 compared with that in group 1. Considering the gestational age at delivery, PMCA expression was increased in group 2 and group 3 compared with that in group 1 after 36 weeks of gestation. SERCA-2 was increased in group 3, but not in group 2 compared with that in group 1 after 36 weeks of gestation. In BeWo cells, MgSO4 treatment increased PMCA and SERCA-2 expression. PMCA expression was influenced by gestational age at delivery, and SERCA-2 expression was increased in the presence of PE and antepartal MgSO4 administration. This indicates that antepartal MgSO4 administration has a greater influence on SERCA-2 than PMCA.

  • calcium
  • plasma membrane Ca2+-ATPase (PMCA)
  • sarcoendoplasmic reticulum CA2+-ATPase
  • pre-eclampsia
  • placentas

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Footnotes

  • Contributors HHC and CRR were responsible for the study concept and design, analysis and interpretation of data, drafting of the manuscript and critical revision of the manuscript for important intellectual content. JRH and JHS were responsible for the acquisition of data. SyO and SJC were involved in the analysis and interpretation of data and statistical analyses. CRR supervised the activities.

  • Funding This research was supported by Kyungpook National University research fund, 2013.

  • Competing interests None declared.

  • Patient consent Obtained.

  • Ethics approval The Institutional Review Board (IRB) for clinical research at Kyungpook National University Hospital in Daegu, Korea, approved the research protocol before beginning this research (IRB No: KNUMC 2016-10-022-001).

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data sharing statement The authors can provide additional unpublished data from the study by email request (chh9861@knu.ac.kr or crroh@skku.edu).

  • Presented at This manuscript was presented as an abstract form at 64th Annual Meeting of the Society for Reproductive Investigation, 15–18 March 2017 in Orlando, FL.

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