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2 Opposite effects of Abcg4 knockout on gonadal fat/body weight ratio changes between male and female mouse littermates
  1. Jinmei Wu1,
  2. Shailendra B Patel2
  1. 1College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China
  2. 2Department of Medicine, Medical College of Wisconsin, Milwaukee, USA

Abstract

Objectives To explore the effect of ATP-binding cassette subfamily G member 4 (Abcg4) gene knockout on gonadal white adipose tissue (GWAT) production in the mouse.

Methods Abcg4 knock-out/GFP knock-in mice were bred and verified by genotyping. Sex-matched littermates 1–2 months old were chosen for the study. The mice were anaesthetized and weighed. Epididymal white adipose tissue (EWAT) from male mice and peri-uterine adipose tissue from female mice was removed and weighed and the gonadal fat/body weight ratio was calculated.

Results The GWAT/body weight ratio of the Abcg4–/– female mouse was 20% higher than that of its wild-type female littermate, but for the male mouse, the ratio was much lower in the knockout mouse than in its wild-type littermate (the ratio for the knockout mouse was only 66% of that of the wild-type littermate). Abcg4 knockout results in an obese female mouse and a lean male mouse, compared to their normal sex-matched littermates.

Conclusions Abcg4 knockout has a remarkable effect on gonadal fat/body weight ratio, with opposite effects in male and female mice.

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