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2nd International Conference on Biomedicine and Pharmaceutics (ICBP) 2014

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PART I: EXTENDED MEETING ABSTRACTS

 

Oral Session

Biomedical Engineering

1

 

EFFECTS OF RADIATION ON APOPTOSIS, ROS AND CELL CYCLE IN BREAST CANCER STME CELLS

Li Yan1, WANG Zhi-cheng2, Qi Yali1*. 1Department of Epidemiology, School of Public Health, Beihua University, Jilin, China. 2Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University, Changchun, China. 1*Department of Epidemiology, School of Public Health, Beihua University, Jilin, China. 195614837@qq.com; 220496586@qq.com. *Corresponding author: E-mail:lhbqyl26@sina.com

Introduction: Cancer stem cells occupy a small portion of whole cancer cells, it has stem cell characteristics, and can immeasurably proliferate, differentiate, and initiate tumorigenesis. In addition, it can cause tumor recurrence, metastasis, and radiotherapy and chemotherapy resistance, its basic mechanism involves some regulation pathways of DNA damage and repair, cell cycle disturbance, autophagy, apoptosis, and cell hypoxia (1-6). Zielske and his colleagues found that after breast cancer radiotherapy, CD44+ CD24 lin phenotype breast stem cells enrich in tumor tissue sample of breast cancer patients, and can cause radioresistance (7). Philips and his colleagues found that oxygen-free radical in breast cancer stem cells was lower than that in breast cancer cells, and 10 Gy irradiation could increase cancer cell oxygen-free radical level, but had no obvious change in cancer stem cells, it indicated that ionizing radiation had more effects on cancer cells than cancer stem cells (8). In order to explore the exact mechanism of radioresistance, in our study, changes of apoptosis, reactive oxygen species (ROS) level, and cell cycle were measured in breast cancer cells and breast cancer stem cells, and provided a new perspective and direction for breast cancer radiotherapy.

Methods: Breast cancer stem cells (MCF-7-S) were sorted by flow cytometry (FCM) with CD44 and CD24 antibodies in breast cancer MCF-7 cells. MCF-7 and MCF-7-S were irradiated with 8 Gy X-rays, after irradiation 4 to 24 hours, apoptotic rate …

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