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Cardiovascular Club I

11:00 AM

Thursday, February 20, 2014


Shao W, Bivona BJ, Kobori H, Navar L. Tulane University School of Medicine, New Orleans, LA.

Purpose of Study: It is known that Ang II activates Rho-kinase activity and Rho-kinase plays an important role in regulating afferent arteriolar tone. However, the role of Rho-kinase on afferent arterioles in chronic Ang II infused hypertensive rats has not been examined. Experiments were performed to determine if Rho-kinase inhibition with fasudil has greater vasodilatory effects on afferent arterioles in Ang II infused rats.

Methods Used: Kidneys from Sprague-Dawley rats were studied in vitro using the blood-perfused juxtamedullary nephron technique.

Summary of Results: Juxtamedullary nephrons were superfused with fasudil (1μM to 100μM) and Ang II (1 nM) in normotensive control rats (n = 6; systolic arterial pressure, 115 ± 1 mmHg) and hypertensive rats infused with Ang II (80ng/min) for 11 to 13 days (n= 5; systolic arterial pressure, 187 ± 6 mmHg). Fasudil alone markedly dilated afferent arterioles in both normotensive and hypertensive rats. During Ang II superfusion, fasudil caused afferent arteriolar vasodilation in both control and Ang II infused rats; however, the afferent arteriolar diameter response to 100 μM of fasudil in Ang II infused rats was significantly greater than in control rats (23 ± 2 vs. 18 ± 1 μm; p < 0.05). Compared with the Ang II superfusion only period, fasudil dilated afferent arterioles similarly in both control and Ang II infused rats (46 ± 6% and 50 ± 12%; p < 0.05). However, the afferent arteriolar diameter responses to 100 μM of fasudil in Ang II infused rats were significantly greater compared with those in control rats (97 ± 18 vs. 68 ± 10%; p < 0.05).

Conclusions: In conclusion, the afferent arteriolar activity of Rho-kinase in Ang II infused hypertensive rats was …

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