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Cardiovascular Club I
Thursday, February 20, 2014
1 INCREASED RHO-KINASE ACTIVITY IN RENAL AFFERENT ARTERIOLES OF ANGIOTENSIN II INFUSED HYPERTENSIVE RATS
Shao W, Bivona BJ, Kobori H, Navar L. Tulane University School of Medicine, New Orleans, LA.
Purpose of Study: It is known that Ang II activates Rho-kinase activity and Rho-kinase plays an important role in regulating afferent arteriolar tone. However, the role of Rho-kinase on afferent arterioles in chronic Ang II infused hypertensive rats has not been examined. Experiments were performed to determine if Rho-kinase inhibition with fasudil has greater vasodilatory effects on afferent arterioles in Ang II infused rats.
Methods Used: Kidneys from Sprague-Dawley rats were studied in vitro using the blood-perfused juxtamedullary nephron technique.
Summary of Results: Juxtamedullary nephrons were superfused with fasudil (1μM to 100μM) and Ang II (1 nM) in normotensive control rats (n = 6; systolic arterial pressure, 115 ± 1 mmHg) and hypertensive rats infused with Ang II (80ng/min) for 11 to 13 days (n= 5; systolic arterial pressure, 187 ± 6 mmHg). Fasudil alone markedly dilated afferent arterioles in both normotensive and hypertensive rats. During Ang II superfusion, fasudil caused afferent arteriolar vasodilation in both control and Ang II infused rats; however, the afferent arteriolar diameter response to 100 μM of fasudil in Ang II infused rats was significantly greater than in control rats (23 ± 2 vs. 18 ± 1 μm; p < 0.05). Compared with the Ang II superfusion only period, fasudil dilated afferent arterioles similarly in both control and Ang II infused rats (46 ± 6% and 50 ± 12%; p < 0.05). However, the afferent arteriolar diameter responses to 100 μM of fasudil in Ang II infused rats were significantly greater compared with those in control rats (97 ± 18 vs. 68 ± 10%; p < 0.05).
Conclusions: In conclusion, the afferent arteriolar activity of Rho-kinase in Ang II infused hypertensive rats was …