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Fasting Glucose Level Modulates Cell Surface Expression of CD11b and CD66b in Granulocytes and Monocytes of Patients With Type 2 Diabetes
  1. Peter Horvath, BS*†,
  2. Stacy R. Oliver, MS*†,
  3. Goutham Ganesan, BS*†,
  4. Frank P. Zaldivar Jr, PhD,
  5. Shlomit Radom-Aizik, PhD,
  6. Pietro R. Galassetti, PhD*†
  1. From the *Department of Pharmacology, School of Medicine, University of California, Irvine, Irvine, CA; and †Institute for Clinical Translational Science, Department of Pediatrics, University of California, Irvine, Orange, CA.
  1. Received October 19, 2012, and in revised form March 18, 2013.
  2. Accepted for publication April 4, 2013.
  3. Reprints: Pietro R. Galassetti, MD, PhD, UCI General Clinical Research Center, Bldg 25, 2nd Floor, 101 The City Drive, Orange, CA. E-mail: pgalasse{at}uci.edu.
  4. Supported by grants NIH CTSA 1UL1TS000153; NIH P01HD048721; NIH K24 DK085223).
  5. PH, SRA, FPZ, and PRG designed the experiments; PH, SRO, and GG performed the experiments; PH and PRG analyzed the data; and PH and PRG wrote the paper.

Abstract

Introduction Cardiovascular complications are the leading cause of mortality in type 2 diabetes (T2DM), in which onset and progression of atherosclerosis is linked to chronic inflammation. Activation status of innate immune cells (granulocytes [Gc], monocytes [Mc]), as reflected by increased CD11b, CD66b, and other surface markers, increases their endothelial and cytokines/chemokines release. Whereas this inflammatory activation seems inversely related to poor glycemic control, the effect of acute spontaneous hyperglycemia on innate immune cell activation remains unclear.

Methods Expression of key markers (CD11b, CD14, CD16, CD62L, and CD66b) was therefore determined by flow cytometry on whole blood of healthy subjects and patients with T2DM with spontaneous fasting euglycemia or hyperglycemia both at baseline and after 30, 90, and 240 minutes of incubation at room temperature.

Results Hyperglycemic patients with T2DM had significantly higher Gc and Mc CD11b and Gc CD66b surface mean fluorescence intensity compared with the euglycemic patients with T2DM whose values were similar to those of the healthy controls. CD16 expression in CD14+CD16+ Mc was elevated in all patients with T2DM, regardless of glycemic levels.

Conclusion Our data suggest that whereas the presence of diabetes per se may have a proinflammatory effect, hyperglycemia seems to further acutely exacerbate innate cell inflammatory status and their consequent endothelial adhesion and vascular damage potential.

Key Words
  • glucose
  • granulocytes
  • monocytes
  • type 2 diabetes

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