Article Text

Download PDFPDF
Lys656Asn Polymorphism of Leptin Receptor Gene Is Related With Leptin Changes After a High Monounsaturated Fat Diet in Obese Patients
  1. Daniel A. de Luis, MD,
  2. Rocio Aller, MD,
  3. Olatz Izaola, PhD,
  4. Rosa Conde, PhD,
  5. Jose Eiros Bouza, MD
  1. From the Center of Investigation of Endocrinology and Clinical Nutrition, Medicine School and Unit of Investigation, Hospital Rio Hortega, University of Valladolid, Valladolid, Spain.
  1. Received August 1, 2012, and in revised form September 6, 2012.
  2. Accepted for publication October 17, 2012.
  3. Reprints: Daniel A. de Luis, MD, Center of Investigation of Endocrinology and Clinical Nutrition. Medicine School, Valladolid University, C/Los perales 16 Simancas 47130, Valladolid, Spain. E-mail: dadluis{at}
  4. D.A.L. performed, designed, and wrote the article. R.A. performed clinical evaluation. O.I. performed the clinical evaluation. R.C. and J.E. performed the laboratory experiments.
  5. This research did not receive any specific grant from any funding agency in the public, commercial or not-for-profit sector.
  6. There is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported.


Background Human obesity is characterized by high levels of leptin, and it has been suggested that obese patients may be leptin resistant. The aim of our study was to investigate the influence of Lys656Asn polymorphism in the leptin receptor gene on metabolic response, weight loss, and serum leptin levels to a high monounsaturated fat hypocaloric diet in obese patients.

Design A sample of 126 obese patients was analyzed in a prospective way. The hypocaloric diet had 1342 kcal, 46.6% of carbohydrates, 34.1% of lipids, and 19.2% of proteins, with a 67.5% of monounsaturated fats, during 3 months.

Results In Lys656Lys genotype, body mass index (−1.8 ± 1.4 kg/m2), weight (−4.3 ± 3.7 kg), fat mass (−3.5 ± 3.5 kg), waist circumference (−5.0 ± 2.9 cm), systolic blood pressure (−3.8 ± 10.5 mm Hg), insulin (−1.5 ± 4.6 mUI/L), and homeostasis model assessment of insulin resistance (−0.4 ± 1.9 Units) decreased. In Asn allele carriers, body mass index (1.5 ± 2.1 kg/m2), weight (−3.7 ± 3.1 kg), waist circumference (−4.4 ± 3.1 cm) decreased. Only leptin levels have a significant decrease in the Lys656Lys group (−6.9 ± 10.1 ng/mL) (24.2%).

Conclusions Patients with Asn656 allele have a different response than Lys656Lys genotype subjects, with a lack of decrease in insulin levels, leptin levels, and homeostasis model assessment of insulin resistance after weight loss.

Key Words
  • Lys656Asn
  • LEPR
  • monounsaturated fat diet
  • leptin

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.