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Do Serum Angiopoietin-1, Angiopoietin-2, and Their Receptor Tie-2 and 4G/5G Variant of PAI-1 Gene Have a Role in the Pathogenesis of Preeclampsia?
  1. Manal Kamal, MD*,
  2. Waleed El-Khayat, MD
  1. From the Departments of *Clinical and Chemical Pathology, and †Obstetrics and Gynecology, Faculty of Medicine, Cairo University, Cairo, Egypt.
  1. Received February 6, 2011, and in revised form June 27, 2011.
  2. Accepted for publication June 30, 2011.
  3. Reprints: Manal Kamal, MD, Department of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt. E-mail: manalmk{at}


Objective To evaluate whether serum angiogenesis markers such as angiopoietins (Ang-1, Ang-2) and their receptor (Tie-2) are altered in women with preeclampsia. We also performed genotyping to determine if the 4G/5G genotypes of −675 PAI-1 gene may play a role in the pathogenesis of preeclampsia.

Materials and Methods Sixty-eight pregnant women with preeclampsia were compared to 35 normotensive pregnant women and 24 normotensive nonpregnant women in a cross-sectional study. Using enzyme-linked immunosorbent assay, levels of serum Ang-1 and Ang-2, and Tie-2 were measured. A single base pair insertion/deletion 4G/5G polymorphism of the PAI-1 gene was determined by polymerase chain reaction.

Results Serum levels of Ang-1 and Tie-2 were significantly different among the study groups (P = 0.001 and P = 0.025, respectively) being lower in the preeclamptic group. Positive significant correlation was found between Ang-2 and Tie-2, (r = 0.26, P = 0.024). The frequency of the genotypes (4G/5G, 4G/4G, and 5G/5G) differed among the groups (P = 0.001). Also, the mean of systolic and diastolic blood pressures differed significantly according to the PAI-1 genotype being higher in those bearing the 4G allele; P = 0.04 and P = 0.023, respectively.

Conclusion Sera Ang-1 and Ang-2, and Tie-2 as well as variants of 4G/5G of PAI-1 polymorphism have positive implications in the pathogenesis of preeclampsia.

Key Words
  • angiopoietins
  • Tie-2
  • PAI-1
  • polymorphism
  • preeclampsia

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