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Western Regional Meeting Abstracts

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WAFMR, WSCI, WAP and WSPR Joint Plenary Session II, 1:45 PM Thursday, January 27, 2011

1 INTRAUTERINE GROWTH RESTRICTION BY UTEROPLACENTAL INSUFFICIENCY AND MATERNAL TOBACCO SMOKING EXPOSURE CAUSES GENDER-SPECIFIC CHANGES IN MRNA EXPRESSION OF HIPPOCAMPAL ESTROGEN RECEPTOR

Numpang B, Caprau D, Ke X, Hale M, Yu X, Jiang C, Kaur R, Fitzhugh M, Callaway C, Joss-Moore L, McKnight R, Lane RH University of Utah, Salt Lake City, UT.

Purpose of Study: 2 major causes for intrauterine growth restriction (IUGR) in the United States are uteroplacental insufficiency (UPI) and maternal tobacco smoking exposure (MTSE). Both UPI and MTSE impacts neurodevelopmental outcomes in offspring born with IUGR, particularly in males. A common characteristic of IUGR animal models is increased hippocampal apoptosis. Neuronal apoptosis is modulated by estrogen signalling - estrogen is anti-apoptotic in the hippocampus. Estrogen mediates it effects on hippocampal apoptosis through estrogen receptors (ER), which are encoded by the genes esr1 and esr2. Currently, little is known about the effects of IUGR by either UPI or MTSE on hippocampal expression of ERs.

Methods Used: IUGR was induced by 2 methods in 2 separate experimental groups: (1) uterine ligation in pregnant rats at day 19 of gestation to mimic UPI, and (2) exposure of pregnant rats to tobacco smoke from day 11 of gestation to term to mimic MTSE. Both groups underwent caesarean section at term. The pups were killed, hippocampi were dissected and RNA extracted. Reverse transcriptase real-time PCR was performed to assay expression of esr1 and esr2.

Summary of Results: UPI decreased esr1 (51% reduction, p=0.01) and esr2 (55% reduction, p=0.02) expression in male pups compared to controls. UPI did not alter esr1 or esr2 expression in female pups. In contrast, MTSE increased esr2 expression (123% increase, p=0.05) in female pups compared to controls, but no changes were seen in males. MTSE did not alter esr1 expression in either gender.

Conclusions: We conclude that the consequence of UPI and MTSE on hippocampal ER mRNA expression is different, despite both UPI and MTSE causing IUGR. The …

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