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DNA Oxidation and Antioxidant Status in Breast Cancer
  1. Solen Himmetoglu, PhD*,
  2. Yildiz Dincer, PhD*,
  3. Yeliz E. Ersoy, MD,
  4. Bariş Bayraktar, MD,
  5. Varol Celik, MD,
  6. Tulay Akcay, PhD*
  1. From the *Department of Biochemistry, and †Department of General Surgery, Istanbul University Cerrahpasa Medical Faculty, Istanbul, Turkey.
  1. Received November 19, 2008, and in revised form April 17, 2009.
  2. Accepted for publication April 28, 2009.
  3. Reprints: Yildiz Dincer, PhD, Dere Sok., Umut Ap. No: 23/44 Sahrayi Cedid, Erenkoy, Istanbul, Turkey. E-mail: stare63{at}
  4. This work was supported by The Research Fund of Istanbul University. Project number BYP/929-17022006 and UDP-2870/27082008.


Purpose Oxidant/antioxidant balance has been suggested as an important factor for initiation and progression of cancer. The objective of this study was to determine 8-hydroxydeoxyguanosine (8-OHdG) level as a marker of oxidative DNA damage, glutathione peroxidase (G-Px), and superoxide dismutase (SOD) activities as antioxidant activity, in sera from women with breast cancer.

Methods Forty-nine patients with malign breast tumor were included in the study. Blood samples were collected before the surgical operation. Serum level of 8-OHdG was measured with a competitive enzyme-linked immunusorbent assay kit, SOD, and G-Px activities were measured by spectrophotometric kits.

Results 8-Hydroxydeoxyguanosine level and SOD activity were found to be increased in breast cancer group as compared with control group. Glutathione peroxidase activity in the breast cancer group was lower than those in the control group. The ratio of 8-OHdG/G-Px in breast cancer patients was found to be higher than those in the controls. There were correlations between 8-OHdG and CA19-9 (r = 0.77; P < 0.01); age and G-Px (r = −0.84; P < 0.05) in the breast cancer group.

Conclusions Data show that serum levels of 8-OHdG and SOD activities are higher in patients with breast cancer. Glutathione peroxidase activity is lower in the breast cancer group. Increased ratio of 8-OHdG/G-Px in breast cancer patients is the evidence for impaired oxidant/ antioxidant balance in breast cancer.

Key Words
  • oxidative DNA damage
  • glutathione peroxidase
  • superoxide dismutase
  • breast cancer
  • 8-hydroxydeoxyguanosine

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