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Detection of α1 Integrin in Urine of Patients With Immunoglobulin A Nephropathy
  1. Jonathan Bank, MD*,
  2. Aharon Ben-David, MD,
  3. Ram Doolman, PhD,
  4. Ben-Ami Sela, PhD,
  5. Ilan Bank, MD
  1. From the *Laboratory for Immunoregulation, the Institutes of †Nephrology and ‡Chemical Pathology, and §Department of Medicine F, The Chaim Sheba Medical Center and Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.
  1. This study was supported by the Kandinoff family grant (I.B.).
  2. The first 2 authors (A.B.-D. and R.D.) contributed equally to this study.
  3. Reprints: Ilan Bank, Department of Medicine F, The Chaim Sheba Medical Center, Tel Hashomer 52621, Israel. E-mail: ibank{at}post.tau.ac.il.

Abstract

Background The α1β1 integrin is a cell surface membrane heterodimer composed of noncovalently linked α1 and β1 polypeptides that is up-regulated on activated and proliferating mesangial cells.

Methods A double-sandwich enzyme-linked immunosorbent assay that detects α1 integrin in a specific and dose-dependent manner at concentrations greater than 150 ng/mL was used to evaluate whether intact α1 polypeptides are secreted in the urine samples of 29 patients with various kidney diseases and in those of 5 healthy individuals.

Results α1 Integrin was detected in 8 of the 29 patients including 3 of 3 patients with biopsy-proven immunoglobulin A nephropathy and 3 of 3 clinically suspected but non-biopsy-proven immunoglobulin A nephropathy with evidence of active nephritis. No α1 integrins were found in samples of 5 healthy controls.

Conclusions α1 Integrin polypeptides can be detected in human urine, particularly in immunoglobulin A nephropathy. Further extensive studies are required to clarify the significance of secretion of α1 integrins in urine of patients with kidney disease.

Key Words
  • integrins
  • VLA-1
  • urine
  • IgA nephropathy
  • α1 integrin

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