Article Text

PDF
B-Cell Kinase Lyn Deficiency in Patients With Systemic Lupus Erythematosus
  1. Stamatis-Nick C. Liossis,
  2. Elena E. Solomou,
  3. Meletios-Athanasios Dimopoulos,
  4. Panayiotis Panayiotidis,
  5. Myron M. Mavrikakis,
  6. Petros P. Sfikakis
  1. From the First Department of Propedeutic Medicine (S.N.C.L, E.E.S., P.P, P.P.S.), Athens University Medical School, Laikon Hospital, Athens, Greece
  2. Department of Therapeutics (M.A.D., M.M.M), Athens University Medical School, Alexandra Hospital, Athens, Greece.
  1. Address correspondence to: Stamatis-Nick C. Liossis, MD, 104 Galatsiou St, GR 11146 Galatsi, Greece. E-mail sliossis{at}hotmail.com
  2. Supported in part by a grant of the Hellenic Society for Rheumatology to S.N.C.L.

Abstract

Background To better understand the molecular background of B-cell overactivity characterizing systemic lupus erythematosus (SLE), we examined the expression of the CD22 co-receptor and of kinase Lyn, which are involved in signaling inhibitory pathways, in B cells from patients with SLE.

Methods Two-color flow cytometry was used to study the expression of surface antigens on freshly isolated peripheral B cells from patients with SLE, disease-control patients, and healthy volunteers. Intracellular kinases Lyn and Syk were analyzed using Western immunoblots, and differences at the messenger RNA (mRNA) level were evaluated using semiquantitative polymerase chain reaction (PCR).

Results Expression of B-cell surface CD22 was intact in patients with SLE, but expression of the B-cell kinase Lyn was significantly decreased in resting, as well as in anti-sIgM-stimulated B-cell-enriched cell lysates obtained from 66% of patients with SLE. Lyn deficiency was disease-specific and unrelated to disease activity. Expression of B-cell kinase Syk was similar in all study groups. Semiquantitative PCR revealed that Lyn mRNA was significantly decreased in lupus patients with decreased Lyn protein expression, suggesting that Lyn deficiency may be caused at least in part by defects at the transcription level.

Conclusions Decreased expression of Lyn in some patients with SLE represents a B-cell defect that may enhance our understanding of SLE molecular pathogenesis by providing rational therapeutic targets.

Key Words
  • B cells
  • systemic lupus erythematosus
  • signal transduction
  • tyrosine kinases
  • Lyn

Statistics from Altmetric.com

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.