Table 3

Summary of antibody dilutions and conditions used in the immunohistochemistry/immunofluorescence analysis of mitochondrial dynamics, mitochondrial biogenesis and synaptic proteins

MarkerPrimary antibody‚ÄĒspecies and dilutionPurchased from (company, location)Secondary antibody, dilution, Alexa fluor dyePurchased from (company, location)
Drp1Rabbit polyclonal 1:300Novus Biological, Littleton, Colorado, USADonkey anti-rabbit IgG Alexa Fluor 488 conjugateThermo Fisher Scientific, Waltham, Massachusetts, USA
Fis1Rabbit polyclonal 1:300Protein Tech Group, Inc, Chicago, Illinois, USADonkey anti-rabbit IgG Alexa Fluor 488 conjugateThermo Fisher Scientific, Waltham, Massachusetts, USA
Mfn1Rabbit polyclonal 1:300Protein Tech Group, Inc, Chicago, Illinois, USADonkey anti-rabbit IgG Alexa Fluor 488 conjugateThermo Fisher Scientific, Waltham, Massachusetts, USA
Mfn2Rabbit polyclonal 1:200Protein Tech Group, Inc, Chicago, Illinois, USADonkey anti-rabbit IgG Alexa Fluor 488 conjugateThermo Fisher Scientific, Waltham, Massachusetts, USA
OPA1Rabbit polyclonal 1:500Novus Biological, Littleton, Colorado, USADonkey anti-rabbit IgG Alexa Fluor 488 conjugateThermo Fisher Scientific, Waltham, Massachusetts, USA
SYNRabbit polyclonal 1:200Protein Tech Group, Inc, Chicago, Illinois, USADonkey anti-rabbit IgG Alexa Fluor 488 conjugateThermo Fisher Scientific, Waltham, Massachusetts, USA
PSD95Rabbit polyclonal 1:400Protein Tech Group, Inc, Chicago, Illinois, USADonkey anti-rabbit IgG Alexa Fluor 488 conjugateThermo Fisher Scientific, Waltham, Massachusetts, USA