Objectives MSD is a type of bio sugar made by an MS bio sugar research institute in South Korea. The bio sugar has a glycan structure with a functional group on the monosaccharide. Our objective was to observe the effects of different concentrations of MSD on toad sciatic nerve-trunk action potential, and whether MSD can improve the inhibitory effect on toad sciatic nerve-trunk action potential of hypertonic glucose solution.
Methods Toad sciatic nerve trunks were randomly divided into three groups, namely a control group, an MSD group and a hypertonic glucose group. Following soaking with different concentrations of MSD, toad sciatic nerve-trunk action potential amplitudes and conduction velocities were determined using a BL-420 biological function experimental system.
Results Compared with the control group, 1% MS inhibited toad sciatic nerve-trunk action potential amplitude and conduction velocity but the effects disappeared after 10 min, while 0.5% MSD decreased action potential amplitude and increased conduction velocity. Soaking in 10% hypertonic glucose with 0.5% MSD after processing permanently inhibited toad sciatic nerve-trunk action potential. MSD can inhibit toad sciatic nerve-trunk action potential amplitude and conduction velocity.
Conclusions The activity of 0.5% MSD is better than that of 1% MSD. MSD can improve the inhibitory effects of 10% hypertonic glucose solution on toad sciatic nerve-trunk action potential.
Acknowledgments This research was financially supported by the National Natural Science Foundation of China (Grant No. 31260207).