Objectives Rana temporaria chensinensis only live in the Changbai mountain area in the northeast of China. The eggs of R. temporaria chensinensis contain many special ingredients that modulate incretion. This study aimed to investigate the effects of R. temporaria chensinensis egg protein hydrolysate on human glioma C6 cell proliferation and apoptosis.
Methods We extracted R. temporaria chensinensis egg protein (500 mg/mL) and investigated its effects on human glioma C6 cell cultures, and subjected it to an MTT assay, colony forming assay, Western blot assay and flow cytometry analysis of apoptosis. We further investigated the effects of R. temporaria chensinensis egg protein hydrolysate (1.5 g/kg) on glioma development and progression in vivo using a mouse model of glioma.
Results R.temporaria chensinensis egg protein hydrolysate inhibited the proliferation of glioma cells; these effects were mediated by the phosphoinositide 3-kinase (PI3K)/AKT signalling pathway.
Conclusions This study suggested that R. temporaria chensinensis egg protein hydrolysate promotes apoptosis of glioma cells both in vitro and in vivo.
Acknowledgments This research was financially supported by Jilin Province Science and Technology Development Program (Grant Nos. 20150309008YY and 20150309009YY).