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Rapid Diagnosis of Mediastinal Tuberculosis With Polymerase Chain Reaction Evaluation of Aspirated Material Taken by Endobronchial Ultrasound–Guided Transbronchial Needle Aspiration
  1. Aysegul Senturk, MD*,
  2. Emine Arguder, MD*,
  3. Habibe Hezer, MD*,
  4. Elif Babaoglu, MD*,
  5. Hatice Kilic, MD*,
  6. Aysegul Karalezli, MD*,
  7. H. Canan Hasanoglu, MD
  1. From the *Department of Pulmonary Diseases, Ankara Ataturk Training and Research Hospital; and †Department of Pulmonary Diseases, Faculty of Medicine, Yildirim Beyazit University, Ankara, Turkey.
  1. Received January 3, 2013, and in revised form April 3, 2014.
  2. Accepted for publication April 8, 2014.
  3. Reprints: Aysegul Senturk, MD, Department of Pulmonary Diseases, Ataturk Training and Research Hospital, Bilkent St Number 3 06800 Ankara, Turkey. E-mail: asenturk1967{at}
  4. There is no conflict of interest.


Background Endobronchial ultrasound–guided transbronchial needle aspiration (EBUS-TBNA) is a diagnostic method for tuberculosis (TB). This study was conducted to determine the efficiency of polymerase chain reaction (PCR) testing for detecting TB lymphadenitis in samples obtained by EBUS-TBNA.

Materials and Methods A total of 93 consecutive patients with hilar/mediastinal lymphadenopathies and diagnosed with granulomatous diseases through histopathological evaluation were included in the study. The specimens provided by EBUS-TBNA were evaluated through pathological, microbiological, and molecular tests.

Results Eighty-nine (95.7%) of the 93 patients had histopathologically granulomatous diseases by EBUS-TBNA. Tuberculosis was diagnosed in 27 (30.3%) patients and sarcoidosis was diagnosed in 62 (69.7%) patients. Four (4.3%) patients were diagnosed through mediastinoscopy. Endobronchial ultrasound–guided transbronchial needle aspiration had an overall diagnostic efficiency in TB of 96.9%, a sensitivity of 90.9%, and a specificity of 100%. Mycobacterium tuberculosis PCR was positive in 17 of the 30 patients. The sensitivity of PCR was 56.7%, the specificity was 100%, and the general efficiency of the test was 96.4%.

Conclusions As a result, the use of M. tuberculosis PCR in the EBUS-TBNA specimens provides a rapid and an accurate diagnosis of TB. Therefore, we recommend the use of M. tuberculosis PCR in the EBUS-TBNA specimens as a rapid diagnostic method for mediastinal lymphadenopathies in patients with suspected TB.

Key Words
  • Mycobacterium tuberculosis polymerase chain reaction
  • endobronchial ultrasound–guided transbronchial needle aspiration

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