Article Text

PDF
131 WNT5A: A POTENTIAL LINK BETWEEN INFLAMMATION AND MORPHOGENESIS IN BRONCHOPULMONARY DYSPLASIA.
  1. M. F. Villosis1,
  2. C. Li1,
  3. M. Durand1,
  4. R. Ramanathan1,
  5. K. Y.C. Kwong1,
  6. P. Minoo.1
  1. 1Division of Newborn Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA.

Abstract

Introduction Wnt5a is a signaling molecule that is expressed in both the mesenchyme and the epithelium of the embryonic lung and plays a key role in its morphogenesis. Recently, Wnt5a was found in adult mononuclear cells. Whether inflammatory cells from the lungs of preterm neonates at risk for BPD also express Wnt5a is unknown.

Objective To examine Wnt5a expression in lung inflammatory cells from preterm neonates.

Methods Inflammatory cells (macrophages and neutrophils) were isolated from lungs of human preterm neonates. Cells were incubated in the presence or absence of LPS and Wnt5a mRNA was measured by real-time RT-PCR.

Results Wnt5a was constitutively expressed by lung inflammatory cells from preterm infants. LPS stimulated Wnt5a mRNA in some but not all samples. The magnitude of response correlated with gestational age, birth weight, and positive endotracheal tube and blood cultures.

Conclusion Expression of Wnt5a by lung inflammatory cells may impact on inflammatory cells functions and/or a morphogenetic impact on lung structural development.

Supported by NHLBI and the Hastings Foundation.

Statistics from Altmetric.com

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.