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504 ANALYSIS OF NUCLEOCYTOPLASMIC DISTRIBUTION OF THE ONCOPROTEIN SKIN IN HUMAN ALVEOLAR RHABDOMYOSARCOMA CELLS BY IMMUNOELECTRON MICROSCOPY.
  1. J. Brown1,
  2. T. Howard1,
  3. B. Reinert1,
  4. D. Bear1
  1. 1Department of Cell Biology and Physiology and Cancer Research and Treatment Center, University of New Mexico School of Medicine, Albuquerque, NM.

Abstract

The Ski oncoprotein functions as a transcriptional corepressor in transforming growth factor β signaling, regulating cellular proliferation, differentiation, and development for several tissues, including skeletal muscle. Multiple human cancers overexpress Ski, which may play a part in malignant cellular transformation. This research focuses on Ski subcellular localization in rhabdomyosarcoma, a pediatric muscle cell cancer. Quantitative analysis of the cellular distribution of the oncoprotein Ski in a rhabodomyosarcoma cell line and a normal human skeletal muscle cell line shows a denser nuclear than cytoplasmic distribution in both cell lines. This analysis shows a significant difference (p = .013) in the nuclear to cytoplasmic ratio of Ski between skeletal muscle cells (mean = 1.38) and rhabodomyosarcoma cells (mean = 2.06). Likewise, there is a significant difference in the fractional distribution to nuclear and cytoplasmic Ski (p = .017), with a skeletal muscle cell line mean of 0.56 and a mean distribution in the rhabdomyosarcoma cell line of 0.65. This indicates that there is a significant quantitative difference between normal skeletal muscle cells and rhabodomyosarcoma cells in the intracellular distribution of the oncoprotein Ski that trends toward nuclear staining.

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