Background The modulated imaging (MI) instrument, recently developed in our laboratory, uses patterned illumination and camera-based detection to obtain quantitative subsurface images of the optical properties of biologic tissues over a wide field-of-view. In this study, we have applied the MI system to associate tumor-induced early angiogenesis with the endogenous optical contrasts such as oxy- ([OHb]), deoxy-([RHb]), and total hemoglobin ([THb]) concentrations and water contents.
Methods 13,762 MAT-III tumor cells were inoculated in the mammary fat pad of female Fischer 344 rats (≈160 g). The MI system acquired the raw images every other day for a week from the breast tissue surface by projecting the seven spatial frequency patterns of NIR light (wavelengths ranging from 650 to 970 nm). From the raw images, the quantification of [OHb], [RHb], [THb], and water contents and the light scattering were carried out and the image maps of respective chromophores were obtained.
Results With the tumor growth, the concentrations of OHb and THb increased and the tissue oxygen saturation also increased whereas the tissue scattering decreased. The representative images are shown below, and the dotted circle shows the approximate site that tumor cells inoculated.
Conclusion Our preliminary results show that the MI system can provide the information on early tumor angiogenesis from the endogenous optical signals. We envision that MI can be a useful tool for tumor therapy monitoring in the future.
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