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Exercise, Caloric Restriction, and Systemic Oxidative Stress
  1. Pietro R. Galassetti,
  2. Dan Nemet,
  3. Andria Pescatello,
  4. Christie Rose-Gottron,
  5. Jennifer Larson,
  6. Dan M. Cooper
  1. From the General Clinical Research Center, University of California Irvine School of Medicine, Irvine, CA
  1. Dr. Galassetti is supported by Career Development Awards from the Juvenile Diabetes Foundation (#11-2003-332) and the National Institutes of Health (K23- RR 18661-01).
  2. Address correspondence to: Dr. Pietro Galassetti, UCI GCRC Bionutrition/Metabolism Core, Bldg 25-2nd Floor, 101 The City Drive, Orange, CA 92868; e-mail: pgalasse{at}


Background In humans, the main sources of reactive oxygen species (ROS), the molecules causing oxidative stress, are mitochondrial superoxide ions and neutrophil-derived oxidative radicals. Circulating antioxidants contribute to the protection against oxidative stress. Although the formation of ROS and secretion of antioxidants are independently regulated by exercise and diet, little is known about their combined effect. We hypothesized that relatively brief, intense exercise training may reduce systemic oxidation via an intrinsic mechanism, independent of changes in circulating antioxidants and of neutrophil-derived enzymes (as may be caused by concomitant caloric restriction).

Methods Nineteen volunteers exercised for 7 days, 3 hours/day at 75% of oxygen uptake. Caloric intake was either 110% of caloric expenditure (high calorie, n = 10) or 75% of caloric expenditure (low calorie, n = 9). Blood samples for F2-isoprostanes, catalase, myeloperoxidase (MPO), interleukin-x (IL-x), white blood cells (WBCs), and other metabolic variables were taken at baseline, at the end of training, and 1 week after completion of the study.

Results Serum F2-isoprostanes (μg/mL), markers of lipid peroxidation, were similarly reduced after 7 days of exercise in the high-calorie (from 35 ± 4 to 27 ± 2) and low-calorie (from 35 ± 3 to 24 ± 2) groups. Similar reductions were observed in IL-x concentrations. Conversely, no change was observed in circulating concentrations of the antioxidant catalase. Whereas total WBCs and neutrophil counts were significantly reduced in the low-calorie group only, no difference in neutrophil-derived MPO was measured between groups.

Conclusion A significant reduction in systemic oxidation may occur relatively early during intense exercise training in healthy young men, independent of caloric intake. The potential contribution to these effects of circulating antioxidants and neutrophil-derived oxidative enzymes will require further investigation.

Key Words
  • oxidative stress
  • F2-isoprostanes
  • lipid peroxidation

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