Background Conventional ventilation (CV) impairs alveolar formation in preterm humans and lambs, whereas nCPAP preserves alveolar formation. 2D protein electrophoresis identified STAT3, a transcriptional factor regulating cell proliferation, and E-cadherin, a cell adhesion molecule, as candidate genes affected by CV. Vitamin A (VitA) supplementation of CV-treated preterm lambs normalizes alveolar formation.
Objective We hypothesized that VitA of the CV-treated preterm lamb would normalize mRNA levels of STAT3 and E-cadherin.
Methods Preterm lambs were treated with CV or nCPAP. Half of the CV lambs were treated with 5,000 U/kg/d of VitA. Ovine STAT3 and E-cadherin cDNAs were cloned and sequenced. Lung mRNA levels of STAT3 and E-cadherin in 3 d preterm lambs treated with CV (n = 3), CV + Vit (n = 4), nCPAP (n = 4), as well as term lamb lungs (n = 4).
Results The STAT3 sequence was 631 bp and demonstrated 94% homology to human STAT3. The E-cadherin sequence was 818 bp and demonstrated 83% homology to human E-cadherin. The following results are presented as percent of nCPAP ± SEM. VitA decreased STAT3 and E-cadherin mRNA levels to 52 ± 14% (p < .05) and 62 ± 15% (p = .09) of nCPAP levels respectively. No significant differences in lung mRNA levels existed for the 2 genes between CV + VitA and normal term lambs.
Conclusion VitA supplementation normalizes mRNA levels of STAT3 and E-cadherin toward the levels found in normal term lambs, which may represent a VitA-induced maturational effect.
Supported by CHRC, HL62875, HL56401, T35 HL07744.
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