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39 SUPPLEMENTAL VITAMIN A NORMALIZES MESSENGER RIBONUCLEIC ACID LEVELS OF STAT3 AND E-CADHERIN IN PRETERM LAMBS TREATED WITH CONVENTIONAL VENTILATON.
  1. K. Au,
  2. R. A. McKnight,
  3. M. J. Dahl,
  4. M. Hale,
  5. C. Callaway,
  6. Q. Fu,
  7. X. Yu,
  8. R. H. Lane,
  9. K. H. Albertine
  1. Pediatrics, University of Utah, Salt Lake City, UT

Abstract

Background Conventional ventilation (CV) impairs alveolar formation in preterm humans and lambs, whereas nCPAP preserves alveolar formation. 2D protein electrophoresis identified STAT3, a transcriptional factor regulating cell proliferation, and E-cadherin, a cell adhesion molecule, as candidate genes affected by CV. Vitamin A (VitA) supplementation of CV-treated preterm lambs normalizes alveolar formation.

Objective We hypothesized that VitA of the CV-treated preterm lamb would normalize mRNA levels of STAT3 and E-cadherin.

Methods Preterm lambs were treated with CV or nCPAP. Half of the CV lambs were treated with 5,000 U/kg/d of VitA. Ovine STAT3 and E-cadherin cDNAs were cloned and sequenced. Lung mRNA levels of STAT3 and E-cadherin in 3 d preterm lambs treated with CV (n = 3), CV + Vit (n = 4), nCPAP (n = 4), as well as term lamb lungs (n = 4).

Results The STAT3 sequence was 631 bp and demonstrated 94% homology to human STAT3. The E-cadherin sequence was 818 bp and demonstrated 83% homology to human E-cadherin. The following results are presented as percent of nCPAP ± SEM. VitA decreased STAT3 and E-cadherin mRNA levels to 52 ± 14% (p < .05) and 62 ± 15% (p = .09) of nCPAP levels respectively. No significant differences in lung mRNA levels existed for the 2 genes between CV + VitA and normal term lambs.

Conclusion VitA supplementation normalizes mRNA levels of STAT3 and E-cadherin toward the levels found in normal term lambs, which may represent a VitA-induced maturational effect.

Supported by CHRC, HL62875, HL56401, T35 HL07744.

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