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88 RADIATION THERAPY POTENTIAL TO OVERCOME TUMOR IMMUNE ESCAPE IN MULTIPLE MYELOMA AFTER STANDARD TREATMENT AND BONE MARROW TRANSPLANTATION.
  1. M Chiriva-Internati1,2,
  2. J Pinkston1,
  3. F Grizzi3,
  4. D'C Nicholas2,
  5. E Cobos2
  1. 1Department of Microbiology and Immunology
  2. 2Internal Medicine, Texas Tech University Health Sciences Center and Southwest Cancer Treatment and Research Center. Lubbock, Texas
  3. 3Scientific Direction, Istituto Clinico Humanitas, IRCCS, Rozzano, Milan, Italy

Abstract

Purpose g-Irradiation of normal cells causes an increased synthesis of specific proteins, including MHC class I/II antigens and ICAM-I. However, few studies have described the effects of high doses of irradiation on the expression of cell surface antigens in tumor cells. The aim of this study was to analyze the effects of high doses of g-irradiation on the surface antigen expression of MHC class I, class II, and ICAM-I in human multiple myeloma (MM) cell lines, including ARP-1, ARK-RS and 10 multiple myeloma primary tumors.

Methods The expression of surface antigens on this MM by FACS analysis at different time points following the exposure to high doses of g-irradiation was documented. The ARP-1, ARK-RS, cells lines, and 10 MM primary tumor cells expressed variable baseline levels of MHC class I/II antigens, ICAM-I.

Summary While doses of 10,000 cGy were not sufficient to totally block cell replication in both cell lines and primary tumors, and exposure to 15,000 was also unable to stop cell replication, only at 18,000 cGy was cell replication completely inhibited. Low doses (10,000 cGy) and lethal doses of irradiation (ie, 15,000 and 18,000 cGy) drastically and consistently increased the expression of all surface antigens present on the cells prior to irradiation.

Conclusion This up-regulation was shown to be dose dependent, with higher radiation doses resulting in higher antigen expression. Furthermore, when the kinetics of this up-regulation were studied 3 and 6 days after irradiation, there was a constant increase in antigen expression in multiple myeloma cells.

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