Article Text

  1. M. Chiriva-Internati1,2,
  2. F. Grizzi3,
  3. E. Cobos2,
  4. E. Frezza4
  1. 1Microbiology and Immunology
  2. 2Internal Medicine
  3. 4Surgery Texas Tech University Health Sciences Center, and Southwest Cancer Treatment and Research Center, Lubbock, TX
  4. 3Scientific Direction, Istituto Clinico Humanitas, IRCCS, Rozzano, Milan, Italy


Purpose SPANX/(CTp11) is a recently identified cancer-testis (CT) antigen in multiple myeloma, melanoma, and other tumors of unrelated histological origin. We here show that it is expressed at mRNA in neoplastic cells from up to 35% of patients affected by liver cancer. Since the expression of this tumor antigen has been demonstrated to be restricted in normal tissues, it seems to be an excellent target for tumor vaccine of liver cancer.

Methods PBMCs were separated from heparinized venous blood by density gradient centrifugation. DCs were differentiated from PBMC.

Results SPANX (CTp11) has been found to be expressed at mRNA level and at protein level by immunohistochemistry in neoplastic cells from up to 35% of patients affected by liver cancer. It contains functional cytotoxic T cell (CTL), supporting its use as a tumor vaccine. In this study, we determined the ability to generate CTp11-specific HLA-class I restricted CTLs from PBL of 5 patients, 3 consecutive SPANX (CTp11)+ patients and two SPANX (CTp11)-patients.

Summary Despite previous chemotherapy and the immunosuppression so often associated with liver cancer, CTL generation was successful in all 3 patients, suggesting the presence in the immune repertoire of SPANX (CTp11)-specific T cells in these patients, irrespective of the SPANX (CTp11) status of their tumors.

Conclusion These observations provide the basis for a clinical study aimed at inducing a cellular immune response directed at SPANX (CTp11)-positive liver cancer patients.

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