Introduction Broadband diffuse optical spectroscopy (DOS) combines multi-frequency domain photon migration (FDPM) with time-independent near-infrared (NIR) spectroscopy to accurately measure bulk tissue absorption and scattering spectra between 600 and 1,000 nm wavelength. Simultaneous determination of absorption and scattering provides the capability for accurate noninvasive direct measurement of tissue metabolite composition and concentrations. DOS could potentially be used to monitor changes in various metabolic solutes such as methemoglobin (MetHb) and methylene blue concentrations during treatment in vivo. The purposes of this study are to evaluate the feasibility of DOS to noninvasively monitor metabolic events during methemoglobinemia and treatment using methylene blue (MB) in vivo and to validate DOS measurements with on-site co-oximetry.
Material and Methods In vivo diagnosis of methemoglobinemia was evaluated in a New Zealand Rabbit model via intravascular injection of sodium nitrite, and the effects were assessed using DOS monitoring with a prototype device constructed in our laboratory. The DOS data were then compared to standard arterial blood measurements of %MetHb using an on-site co-oximeter.
Results In vivo MetHb concentration was quantitatively measured by DOS and was compared to %MetHb obtained through arterial blood from an on-site co-oximetry. %MetHb values from DOS and co-oximetry showed a close correlation (r 2 = .902, p < .0001, N = 4) and the quantification of %MetHb by DOS was not influenced by the cross-talk from oxy- and deoxyhemoglobin concentrations (as demonstrated by varying arterial and venous oxygenation).
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