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Parameters for Measurement of Oxidative Stress in Diabetes Mellitus: Applicability of Enzyme-Linked Immunosorbent Assay for Clinical Evaluation
  1. Eisei Noiri,
  2. Hirokazu Tsukahara
  1. From the Department of Medicine (E.N.), Faculty of Medicine, Center for Dialysis and Applied Medicine, University of Tokyo, Tokyo, Japan; and Department of Pediatrics (H.T.), Faculty of Medicine, Fukui University, Fukui, Japan. Part of this study was supported by grants to Dr. Noiri from the Cell Science Research Foundation (Osaka, Japan) and Fukuda Foundation for Medical Technology (Tokyo, Japan).
  1. Address correspondence to: Dr. Eisei Noiri, Department of Medicine/Nephrology 107 Laboratory, University of Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-8655, Japan; e-mail: noiri-tky{at}


Investigations of the mechanisms involved in the onset and progression of diabetes have recently confronted the role of reactive oxygen species (ROS) and oxidative stress. Prolonged exposure to hyperglycemic conditions induces nonenzymatic glycation of protein via the so-called Maillard reaction, resulting in Schiff-base products and Amadori products that engender ROS production. These processes initiate and exacerbate micro- and macrovascular complications in diabetes. Increased oxidative stress is induced by excessive ROS production and inadequate antioxidant defenses. Recently, oxidative stress status markers have been associated directly with the severity and prognosis of diabetes. To examine oxidative stress, reliable and high-throughput methods are needed to examine large numbers of clinical samples. The emerging availability of enzyme-linked immunosorbent assay (ELISA) for oxidative stress status markers allows its application to assessment of various pathophysiologic conditions, including diabetes. This review outlines the recent achievements of ELISA application for clinical studies elucidating oxidative stress. It introduces the potential applicability of ELISA for investigating oxidative stress in diabetes.

Key Words
  • enzyme-linked immunosorbent assay
  • diabetes mellitus
  • advanced glycation end products
  • oxidative stress
  • lipid peroxide
  • nitric oxide
  • peroxynitrite
  • thioredoxin
  • fatty acid binding protein

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