Article Text

  1. A. Fabre,
  2. L. Blacklock,
  3. K. Crookston,
  4. E. Libby,
  5. D. Garcia
  1. Albuquerque, NM.


Background Cross-linked fibrin degradation products known as d-dimers are commonly used to evaluate the likelihood of thromboembolism in clinical and research settings. Currently, frozen samples of platelet-poor plasma are stored at -70 degrees C for later use in research and quality-control. Demonstration of d-dimer stability in plasma stored at -20 C could diminish the cost of storing these samples.

Purpose The purpose of this study was to determine whether d-dimer concentration is as stable at -20 C as at -70 C, when stored for as long as 6 months.

Methods Citrated platelet-poor plasma samples were collected from 20 male and 16 female asymptomatic volunteers, and baseline d-dimer concentrations were determined using the STA®-Liatest® D-Di. Aliquots from these 36 samples were stored at -20 C and -70 C for d-dimer measurement at 1, 3, and 6 months after collection. Comparison of d-dimer level was done by subtracting the baseline value for each individual from their subsequent observed values. Each individual's changes from baseline were then analyzed using paired Student's t-test, repeated measures analysis of variance, or multiple linear regression as appropriate.

Results There were 216 samples; d-dimer concentrations ranged from 0.01 to 1.08 mcg/ml. The changes in d-dimer difference over time were not significantly different for the two storage temperatures (p=0.29). The observed differences were small and unlikely to be of clinical importance. (Table)

Conclusion This study demonstrates that platelet-poor plasma can be stored for up to 6 months at -20 C rather than -70 C without significantly altering the stability of d-dimer concentration as measured by the STA®-Liatest® D-Di.

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