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Neutrophil Adhesion Molecule Expression in Familial Mediterranean Fever: Discordance between the Intravascular Regulation of β2 Integrin and L-Selectin Expression in Acute Attack
  1. Yair Molad,
  2. Amir Fridenberg,
  3. Konstantine Bloch,
  4. Pnina Langevitz,
  5. Masza Mukamel,
  6. Jaqueline Sulkes,
  7. Mordechai Pras,
  8. Avi Livneh
  1. From the Laboratory of Inflammation Research (Y.M., A.F., K.B.), Felsenstein Medical Research Center, and Rheumatology (Y.M.) and Epidemiology Units (J.S.), Rabin Medical Center, Beilinson Campus, Petah Tiqva, Israel; Pediatric Rheumatology Unit (M.M.), Schneider Children's Medical Center of Israel, Petah Tiqva, Israel; Department of Medicine F (PL., M.P., A.L.), Sheba Medical Center, Tel Hashomer, Israel; and Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.
  1. Address correspondence to: Dr. Yair Molad, Rheumatology Unit, Rabin Medical Center, Beilinson Campus, Petah Tiqva 49 100, Israel; E-mail: ymolad{at}clalit.org.il.

Abstract

Background To determine the surface expression of neutrophil β2 integrin (CD11b/CD18) and L-selectin (LS) adhesion molecules in patients with familial Mediterranean fever (FMF) and to investigate the in vitro regulation of their expression in response to chemoattractant stimuli.

Methods Neutrophil surface expression of CD11b and LS molecules was analyzed by flow cytometry in anticoagulated whole blood drawn from FMF patients and normal controls, and the in vitro regulation of these molecules induced by the chemoattractant N-formyl-methionyl-leucyl-phenylalanine (FMLP) was assayed.

Results Patients during acute FMF attacks showed a statistically significant increased neutrophil surface CD11b compared with normal controls (mean fluorescence intensity: 22.8 ± 13.7 vs 12.8 ± 10.41, respectively; p = .03). There was no difference in LS expression between the groups. Neutrophils of FMF patients regulate CD11b and LS expression induced by chemoattractant (FMLP) stimulation to a degree similar to that in controls.

Conclusions β2 Integrin is up-regulated during an acute attack of FMF in dissociation with LS expression, suggesting a unique nonchemoattractant-mediated neutrophil activation.

Key Words
  • β2 integrin
  • L-selectin
  • familial Mediterranean fever
  • neutrophil

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